Avian leukosis virus (ATCC® VR-658)

Classification: Retroviridae, Alpharetrovirus, Avian leukosis virus (Subgroup B)  /  Product Format: frozen

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Classification Retroviridae, Alpharetrovirus, Avian leukosis virus (Subgroup B)
Deposited As Rous associated virus-B
Agent Avian leukosis virus
Strain RAV-2
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
tissue, animal
Isolation date: 1965
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Grows more slowly than RAV-1.
Effect on Host
No, in vitro effects: Cytopathic effects in TC. Detected by gs antigen or helper activity
No CPE in TC. Detected by gs antigen or helper activity. Lymphoid leukosis in chickens.
Yes, in vivo effects: Lymphoid leukosis in chickens
Recommended Host

Recommended Host:  primary chicken embryo fibroblasts cells of phenotype C/O (chick cells not resistant to any subgroup of avian sarcoma/leucosis virus)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 7 to 22 days; For best results cells should be 70-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
No, in vitro effects: Cytopathic effects in TC. Detected by gs antigen or helper activity
No CPE in TC. Detected by gs antigen or helper activity. Lymphoid leukosis in chickens.
Yes, in vivo effects: Lymphoid leukosis in chickens
Name of Depositor W Okazaki, HG Purchase, Holdenried, E Berstein
Special Collection ATCC
Source
tissue, animal
Isolation date: 1965
Year of Origin 1965
References

Morgan HR, Traub W. Natl. Cancer Inst. Monogr. 17: 392, 1964.

Vogt PK. Envelope classification of avian RNA tumor viruses. Bibl. Haematol. 36: 153-167, 1970. PubMed: 4376359

Vogt PK. A heterogeneity of Rous Sarcoma Virus revealed by selectively resistant chick embryo cells. Virology 25: 237-247, 1965. PubMed: 14297211

or colder

chicken

There are some primary chicken fibroblasts that can't be infected by avian retroviruses including RAV-2. Cells from chickens of type C/O, C/A and C/BC are reported to be susceptible.

or colder

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation