Avian leukosis virus (ATCC® VR-658)

Classification: Retroviridae, Alpharetrovirus, Avian leukosis virus, Subgp.B  /  Product Format: frozen

Permits and Restrictions

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Classification Retroviridae, Alpharetrovirus, Avian leukosis virus, Subgp.B
Agent Avian leukosis virus
Strain RAV-2
Biosafety Level 2
Isolation
tissue, animal
Isolation date: 1965
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Grows more slowly than RAV-1.
Effect on Host
No, in vitro effects: Cytopathic effects in TC. Detected by gs antigen or helper activity
No CPE in TC. Detected by gs antigen or helper activity. Lymphoid leukosis in chickens.
Yes, in vivo effects: Lymphoid leukosis in chickens
Recommended Host

Recommended Host:  primary chicken embryo fibroblasts cells of phenotype C/O (chick cells not resistant to any subgroup of avian sarcoma/leucosis virus)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 7 to 22 days; For best results cells should be 70-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
No, in vitro effects: Cytopathic effects in TC. Detected by gs antigen or helper activity
No CPE in TC. Detected by gs antigen or helper activity. Lymphoid leukosis in chickens.
Yes, in vivo effects: Lymphoid leukosis in chickens
Name of Depositor W Okazaki, HG Purchase, Holdenried, E Berstein
Special Collection ATCC
Source
tissue, animal
Isolation date: 1965
Year of Origin 1965
References

Morgan HR, Traub W. . Natl. Cancer Inst. Monogr. 17: 392, 1964.

Vogt PK. Envelope classification of avian RNA tumor viruses. Bibl. Haematol. 36: 153-167, 1970. PubMed: 4376359

Vogt PK. A heterogeneity of Rous Sarcoma Virus revealed by selectively resistant chick embryo cells. Virology 25: 237-247, 1965. PubMed: 14297211

or colder

chicken

There are some primary chicken fibroblasts that can't be infected by avian retroviruses including RAV-2. Cells from chickens of type C/O, C/A and C/BC are reported to be susceptible.

or colder

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation