Adeno-associated virus 1 (ATCC® VR-645™)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus-1  /  Product Format: frozen

Classification Parvoviridae, Dependovirus, Adeno-associated virus-1
Agent Adeno-associated virus 1
Strain none
Applications
Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells.
Biosafety Level 2
Isolation
Pool of RhMk TC infected with SV-15 virus
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Depends upon adenovirus for replication. Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. *Helper virus may be inactivated by heating to 56C for 30 min.
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  HEK-293 (ATCC® CRL-1573TM)
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 2-4 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Name of Depositor WP Parks
Special Collection ATCC
Source
Pool of RhMk TC infected with SV-15 virus
References

Atchison RW, et al. Adenovirus-associated defective virus particles. Science 149: 754-756, 1965. PubMed: 14325163

or colder

or colder