Gallid herpesvirus 2 (ATCC® VR-624)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus
Agent Gallid herpesvirus 2
Strain GA-5
Biosafety Level 2
chicken tissue with Marek's disease
Product Format frozen 1 mL per vial
Storage Conditions Vapor phase of liquid nitrogen
Infected CK cell suspension with 7% DMSO + Penicillin/Streptomycin + Fungizone®. Similar to JM strain. Dilution of infected chicken kidney cell suspension with medium 2-3 times the volume after thawing will enhance virus recovery.
Effect on Host
CPE, focal lesions
Recommended Host
chicken kidney cells
Growth Conditions
Temperature: 38.5°C
Incubation: 5-7 days
Effect on Host
CPE, focal lesions
Name of Depositor BW Calnek, KA Schat, Cornell University New York State College of Veterinary Medicine
Chicken tissue with Marek?s disease, Georgia, 1964

Calnek BW, Hitchner SB. Highly potent, viable and stable cell-free virus preparations from cells infected with cell-associated viruses and method for obtaining the same. US Patent 3,783,098 dated Jan 1 1974

Eidson CS, Schmittle SC. Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis. 12: 467-476, 1968. PubMed: 4301071

Reddy SM, et al. Development of a quantitative-competitive polymerase chain reaction assay for serotype 1 Marek's disease virus. Avian Dis. 44: 770-775, 2000. PubMed: 11195630.

Silva RF. Differentiation of pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDVs) by the polymerase chain reaction amplification of the tandem direct repeats within the MDV genome. Avian Dis. 36: 521-528, 1992. PubMed: 1329706

Cross References

Nucleotide (GenBank) : U63464 Gallid herpesvirus 2 DNA polymerase gene, partial cds.