Gallid herpesvirus 2 (ATCC® VR-585)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Classification Herpesviridae, Mardivirus
Agent Gallid herpesvirus 2
Strain JM
Biosafety Level 2
Isolation
chicken tumor; blood
Product Format frozen
Storage Conditions   Vapor phase of liquid nitrogen
Comments
Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens.
Stable for at least 2-3 years in viable cells frozen at -196C.
Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens.
Effect on Host
Yes, in vitro effects: Cytopathic effects (herpesvirus-like CPE) in tissue culture
C:  Marek’s disease lesions;  CAM:  pocks;          TC:  herpesvirus-like CPE
Yes, in vivo effects: Marek's disease lesions in chickens
Yes, in vivo effects: pocks on chorioallantoic membrane in eggs
Recommended Host
chicken, i.p. inoculation; chicken kidney cells; duck embryo fibroblasts

Host of Choice:  C (i.p.),  CAM, CK, DEF (ATCC

        CCL-141)

Host Range:  C (i.p.), CAM, CK, DEF

chicken, i.p. inoculation; chorioallantoic membrane; chicken kidney cells; duck embryo fibroblasts
Growth Conditions
Temperature: 37.0°C
Duration: 7-14 days
Effect on Host
Yes, in vitro effects: Cytopathic effects (herpesvirus-like CPE) in tissue culture
C:  Marek’s disease lesions;  CAM:  pocks;          TC:  herpesvirus-like CPE
Yes, in vivo effects: Marek's disease lesions in chickens
Yes, in vivo effects: pocks on chorioallantoic membrane in eggs
Name of Depositor RL Witter
Special Collection ATCC
Source
chicken tumor; blood
References

Sevoian M. . Vet. Med. 57: 500, 1962.

Solomon JJ, et al. Studies on the etiology of Marek's disease. I. Propagation of the agent in cell culture. Proc. Soc. Exp. Biol. Med. 127: 173-177, 1968. PubMed: 5644639

Stephens EA, et al. Characteristics of JMV Marek's disease tumor: a nonproductively infected transplantable cell lacking in rescuable Virus. J. Natl. Cancer Inst. 57: 865-874, 1976. PubMed: 187779