H-1 parvovirus (ATCC® VR-356)

Classification: Parvoviridae, Parvovirinae, Parvovirus  /  Product Format: frozen 1.0 mL per vial

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Classification Parvoviridae, Parvovirinae, Parvovirus
Deposited As H-1 virus
Agent H-1 parvovirus
Strain Toolan
Applications
Produces deformity or death in newborn hamsters and neutralizing antibodies in humans, monkeys, hamsters and rats.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions  -70°C or colder
Genome Sequenced Strain

Parvovirus h-1, complete genome

Comments
Plaque purified on Salk MkHe.  Hemagglutinates a variety of RBC, particularly guinea pig, hamster and human, and also has a CF antigen.  Produces deformity or death in newborn hamsters and neutralizing antibodies in humans, monkeys, hamsters and rats.
Effect on Host
Cytopathic effects (cell death) in tissue culture; death or deformity of newborn hamster depending on virus concentration
Recommended Host
Chang L cells (ATCC® CCL-13™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection:  Plant cells 24-48 hours in advance and infect when cultures are about 70% confluent.   Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1.  Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.

Incubation: Incubate infected culture for 5 days at 37°C temperature in a humidified 5% COatmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
Cytopathic effects (cell death) in tissue culture; death or deformity of newborn hamster depending on virus concentration
Name of Depositor HW Toolan
Source Human embryo lung (4 months operative abortion) from mother with metastatic breast cancer, who had an HAI and neutralizing titer of 1:640 to H-1 virus.
References

Toolan H, Ledinko N. Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208: 812-813, 1965. Pubmed: 4958078

Karasaki S. Size and ultrastructure of the H-viruses as determined with the use of specific antibodies. J Ultrastruct Res 16(1): 109-122, 1966. Pubmed: 4959542

Proc. Am. Assoc. Cancer Res. 5: 64, 1964.

Rhodes SL, Paradiso PR. Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation. J Virol 45(1): 173-184, 1983.

Cross References

Nucleotide (GenBank) : X01457.1 Parvovirus h-1, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation