Vaccinia virus ts mutant (ATCC® VR-3163)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts67
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Biosafety Level 2
Isolation
This conditional lethal mutant, originally designated plaque isolate number 8125, was isolated from the IHD-W strain of Vaccinia by S. Dales, selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. This mutant was assigned to Dales EM category E, inducing foci of viroplasm, aberrant membranes without spicules, and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions. The non-permissive incubation temperature is 39.5oC.
Product Format frozen
Storage Conditions -70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate number 8125, was isolated from the IHD-W strain of Vaccinia by S. Dales, selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. This mutant was assigned to Dales EM category E, inducing foci of viroplasm, aberrant membranes without spicules, and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions. The non-permissive incubation temperature is 39.5oC.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Growth Medium Medium 30-2002: Dulbecco's Modified Eagle's Medium (DMEM)
Fetal Bovine Serum
Recommended Host

Recommended Host: BSC-40 (ATCC CRL-2761)

Production host: BSC-40 (ATCC CRL-2761) Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Name of Depositor S. Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm