Vaccinia virus ts mutant (ATCC® VR-3158)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts91
Applications
Non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category E, Q. This mutant was eliminated during Complementational analysis.
Biosafety Level 2 BSL 2Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses
Isolation
This conditional lethal mutant, originally designated plaque isolate no. 9251, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category E, Q. This mutant was eliminated during Complementational analysis.
Effect on Host
Cytopathic effects (CPE): enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Growth Medium Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 5% CO2
Effect on Host
Cytopathic effects (CPE): enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Name of Depositor S dales, R Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation