Vaccinia virus ts mutant (ATCC® VR-3158)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts91
Applications
Non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category E, Q. This mutant was eliminated during Complementational analysis.
Biosafety Level 2 BSL 2Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This conditional lethal mutant, originally designated plaque isolate no. 9251, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category E, Q. This mutant was eliminated during Complementational analysis.
Effect on Host
Cytopathic effects (CPE): enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 5% CO2
Effect on Host
Cytopathic effects (CPE): enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in cell culture include cell rounding and death, with eventual sloughing
Name of Depositor S dales, R Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation