Vaccinia virus ts mutant (ATCC® VR-3146)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts30
Applications
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D4, encoding a viral uracyl DNA glycosylase.
Biosafety Level 2
Isolation
This conditional lethal mutant, originally designated plaque isolate no. 4149, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B indicating that it induces foci of a fibrous viroplasmic matrix substance in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D4, encoding a viral uracyl DNA glycosylase.
Product Format frozen
Storage Conditions -70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate no. 4149, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B indicating that it induces foci of a fibrous viroplasmic matrix substance in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D4, encoding a viral uracyl DNA glycosylase.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Growth Medium Fetal Bovine Serum
ATCC® Medium 30-2002: Dulbecco's Modified Eagle's Medium (DMEM)
Recommended Host

Recommended Host: BSC-40 (ATCC CRL-2761)

Production host: BSC-40 (ATCC CRL-2761) Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Name of Depositor S. Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm