Hepatitis A virus (ATCC® VR-2281)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen

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Classification Picornaviridae, Hepatovirus
Deposited As Hepatitis A
Agent Hepatitis A virus
Strain HAS-15
Applications
Enteric Research
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Images ATCC VR-2281 micrograph images
Comments
Genome contains 18-nucleotide deletion between VPI nucleotides 3383-2301. Attenuated for chimpanzees at 200 ng dose.
Effect on Host
Gradual deterioration of cells; titer by ELISA or by RT-PCR (cells become persistently infected). Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate, but remain attached indicating persistent infection.
Recommended Host
FRhK-4 (ATCC® CRL-1688™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 14-21 days at 37°C in a humidified 5% CO2 atmosphere.
Effect on Host
Gradual deterioration of cells; titer by ELISA or by RT-PCR (cells become persistently infected). Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate, but remain attached indicating persistent infection.
Name of Depositor BH Robertson
Source Human feces
References

Robertson BH, et al. Large scale production of hepatitis A virus in cell culture: effect of type of infection on virus yield and cell integrity. J. Gen. Virol. 69: 2129-2134, 1988. PubMed: 2841419

Cross References

Nucleotide (GenBank) : JQ425480.1 Hepatitis A virus strain HAS-15, complete genome

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