Avian coronavirus (ATCC^® VR-22^™)

Avian coronavirus ATCC^® VR-22^™

frozen

For-Profit: $516.00 Non-Profit: $430.00

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Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Coronaviridae, Gammacoronavirus
Agent	Avian coronavirus
Strain	Egg-adapted (Michigan State Univ. Repos. Code 42)
Common Name	Infectious bronchitis virus (IBV)
Applications	For passage in eggs, clarify sample and use supenatant fluid for inoculum via allantoic cavity.
Biosafety Level	2
Isolation	Specimen from chicks with naturally occurring disease, New Jersey, 1935
Product Format	frozen
Storage Conditions	-70°C or colder

Comments	Reported to cause syncytial CPE in CEK TC with subsequent necrosis of cells. For passage in eggs, clarify sample and use supenatant fluid for inoculum via allantoic cavity. Chill eggs for harvest when one or two enbryos are dead and the remainder are week or sluggish.
Effect on Host	Yes, in vivo effects: death of chicken embryo Death of CE Yes, in vitro effects: syncytia in chicken embryo kidney tissue culture

Recommended Host	10 day chicken embryo, i.a. inoculation Host of Choice: CE (10 day, i.a.) Host Range: CE, CAM and CEK TC chicken embryo (chorioallantoic membrane); chicken embryonic kidney cells in tissue culture
Growth Conditions	Duration: 1-2 days at 37C, via allantoic route

Effect on Host	Yes, in vivo effects: death of chicken embryo Death of CE Yes, in vitro effects: syncytia in chicken embryo kidney tissue culture

Name of Depositor	CH Cunningham
Special Collection	ATCC
Source	Specimen from chicks with naturally occurring disease, New Jersey, 1935
Year of Origin	1935
References	Cunningham CH. . Adv. Vet. Sci. 14: 106-148, 1970. McIntosh K. . Curr. Top. Microbiol. Immunol. 63: 86-119, 1974. Beaudette FR. . J. Am. Vet. Med. Assoc. 90: 51, 1937.

Permits

These permits may be required for shipping this product:

Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation