Canine coronavirus (ATCC® VR-2068)

Classification: Nidovirales, Coronaviridae, Coronavirus, Group 1  /  Product Format: frozen

Classification Nidovirales, Coronaviridae, Coronavirus, Group 1
Agent Canine coronavirus
Strain CCV-TN449
Common Name Canine coronavirus
Applications
Respiratory research
Biosafety Level 2
Isolation
Dog which died of gastroenteritis; propagated and attenuated in CRFK cell cultures
Product Format frozen
Storage Conditions -70°C or colder
Effect on Host
Yes, in vitro effects: Visualize by immunofluorescence
CPE, syncytial fusing of cells
Recommended Host
CRFK cells (ATCC CCL-94); Woods feline kidney cells; canine kidney cells

Recommended Host: CRFK (ATCC® CCL-94TM)

Growth Conditions
Temperature: 35°C to 38°C
Incubation: 1 to 4 days at 37°C, rocking at setting #1, a 5%CO2 in air atmosphere is recommended.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Effect on Host
Yes, in vitro effects: Visualize by immunofluorescence
CPE, syncytial fusing of cells
Name of Depositor American Home Products Corp.
Special Collection ATCC
Source
Dog which died of gastroenteritis; propagated and attenuated in CRFK cell cultures
References

Acree WM, et al. Adjuvants for vaccines. US Patent 5,047,238 dated Sep 10 1991

Acree WM, et al. Inactivated canine coronavirus vaccine. US Patent 4,567,042 dated Jan 28 1986

Acree WM, et al. Canine corona virus vaccine. US Patent 4,567,043 dated Jan 28 1986

Acree WM, et al. Canine corona virus vaccine. US Patent 4,824,785 dated Apr 25 1989

Acree WM, Black JM. Canine corona virus vaccine. US Patent 5,013,663 dated May 7 1991