Feline calicivirus (ATCC® VR-2057)

Classification: Caliciviridae, Vesivirus  /  Product Format: frozen 1.0 mL

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Classification Caliciviridae, Vesivirus
Agent Feline calicivirus
Strain FCV-2280
Applications
The virus is not neutralized by FCV-M8 antisera.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Genome Sequenced Strain

Feline calicivirus strain FCV-2280, complete genome

Images ATCC VR-2057 images
Comments
FCV-2280 is not neutralized by antiserum to the vaccine strains FCV-M8, FCV-F9 and FCV-255.  This virus has been adapted to tissue culture propagation and may not readily infect cats.  Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe on the patent claims.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
Fcwf-4 cells (ATCC® CRL-2787™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, cell rounding and sloughing
Name of Depositor University of California, Davis
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Special Collection Patent
Source Blood from naturally infected cat
References

Pedersen NC, Laliberte L, and Ekman S. A transient febrile limping syndrome of kittens caused by two different strains of feline calicivirus. Feline Pract 13(1): 26-35, 1983.

Pedersen NC. Feline calicivirus vaccine. US Patent 4,522,810 dated Jun 11 1985

Cross References

Nucleotide (GenBank) : KC835209.1 Feline calicivirus strain FCV-2280, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation