Vaccinia virus (ATCC® VR-2029)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain vP-6
Applications
produces protein thymidine kinase
Biosafety Level 2
Isolation
Two copies of the herpes virus BamHI fragment coding for TK were cloned (head to tail) into the BamHI site of the vaccinia HindIII F-fragment. This construct was then cloned into VTK-11 by in vivo recombination.
Product Format frozen
Storage Conditions -70°C
Comments
A recombinant virus consisting of a TK -vaccinia (VTK-11) with an HSV-TK insert which is expressed.
Two copies of the herpes virus BamHI fragment coding for TK were cloned (head to tail) into the BamHI site of the vaccinia HindIII F-fragment. This construct was then cloned into VTK-11 by in vivo recombination.
Effect on Host
Cytopathic effects in tissue culture
Recommended Host
HeLa cells (ATCC CCL-2); CV-1 cells (ATCC CCL-70)
Growth Conditions
Incubation: 5 days
Effect on Host
Cytopathic effects in tissue culture
Name of Depositor E Paoletti
References

Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986

Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988

Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
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