Gallid herpesvirus 3 (ATCC® VR-2001)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Permits and Restrictions

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Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus type 2
Agent Gallid herpesvirus 3
Strain SB-1 (clone of SB)
Applications
NOTE: This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims.
Will protect against MDV and non-virus producing JM tumor transplant.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
chicken (S-strain) peripheral blood lymphocytes
Product Format frozen
Storage Conditions  Vapor phase of liquid nitrogen
Comments
Non-oncogenic, cell-associated. Will protect against MDV and non-virus producing JM tumor transplant. NOTE: This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims.
Note that SB-1 is strictly cell-associated, damage to the infected cells will cause loss of titer. Thaw frozen ampule in 27C waterbath; dilute content slowly in 9.0 ml medium or PBS; pellet cells (10min at 400Xg); resuspend in culture medium.
Effect on Host
 CPE, cell rounding and syncytia formation
Recommended Host
chicken embryo fibroblasts
Growth Conditions
Temperature: 38°C to 39°C
Incubation:  Incubate infected culture for 7 days at 38-39°C in a humidified 5% CO2 atmosphere, until CPE are well advance throughout.

Cryopreservation
Storage temperature: Liquid nitrogen
Effect on Host
 CPE, cell rounding and syncytia formation
Name of Depositor BW Calnek
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Source
chicken (S-strain) peripheral blood lymphocytes
References

Schat KA, Calnek BW. Characterization of an apparently nononcogenic Marek's disease. J. Natl. Cancer Inst. 60: 1075-1082, 1978. PubMed: 76680

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation