Vaccinia virus (ATCC® VR-1774)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen 1 mL per vial

Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain CL
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions  -70°C or colder
Comments
VR-1774 is produced in BSC-40 cells, which is a change in cell line from ATCC VR-117 to increase titer.
Effect on Host
 CPE, refractile cell rounding
CPE, refractile cell rounding
Recommended Host

Production Host:  BSC-40 (ATCC® CRL-2761)

BSC-40 (ATCC CRL-2761)
Growth Conditions
Temperature: 37°C
Growth Conditions: Plate cells 24-48 hours in advance and infect when cultures are 90 to 95% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 30 minutes at 37°C in a humidified 5% CO2 atmosphere, rocking every 7-10 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host
 CPE, refractile cell rounding
CPE, refractile cell rounding
Name of Depositor F Fenner, Australian National University
Source
Most likely derived from New York City Department of Health strain of vaccinia virus. Cell culture adapted to BSC-40 cells.
References

Fenner, F and Burnet, FM. A short description of the Poxvirus group (Vaccinia and related viruses). Virology 4: 305-314, 1957. PubMed: 13496547.

Craigie J. . Br. J. Exp. Pathol. 13: 259, 1932.

Parker RF, et al. Further studies of the infectious unit of Vaccinia. J. Exp. Med. 74: 263-281, 1941.