Vaccinia virus (ATCC® VR-1605)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain rVV-hgp100
Applications
VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI.
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Comments
VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI.
Effect on Host
Cell enlargement and clustering of rounded cells in cell culture; plaques with rounding and eventual sloughing.
Recommended Host
Production Host: Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts)).

Production Host:  Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts).)

Alternate Hosts: BS-C-1 cells (ATCC CCL-26), Vero cells (ATCC CCL-81), and HeLa cells (ATCC CCL-2)
Growth Conditions
Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Process virus by freeze/ thawing it three times by vapor phase of liquid nitrogen and 37.0°C water bath (vortex mixing on high speed for 20 seconds between each freeze/ thaw), sonicating with 40% power for 30 seconds, and then a final vortex mixing on high speed for 30 seconds. Remove cell growth medium from the flask, and inoculate with a small volume of virus stock diluted in virus growth medium to provide a MOI of about 1-3. Adsorb 1-2 hours at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubate infected culture for 1-3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
Cell enlargement and clustering of rounded cells in cell culture; plaques with rounding and eventual sloughing.
Name of Depositor Dr. Zhiya Yu from Surgery Branch/ NCI/ NIH
Source
rVV-hgp100 was constructed in Dr. Nicolas P. Restifo's laboratory of Surgery Branch/ NCI/ NIH in the early 1990's.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation