Vaccinia virus (ATCC® VR-1605)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain rVV-hgp100
Applications
VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI.
Effect on Host
Cell enlargement and clustering of rounded cells in cell culture; plaques with rounding and eventual sloughing.
Recommended Host
Production Host: Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts)).

Production Host:  Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts).)

Alternate Hosts: BS-C-1 cells (ATCC CCL-26), Vero cells (ATCC CCL-81), and HeLa cells (ATCC CCL-2)
Growth Conditions
Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Process virus by freeze/ thawing it three times by vapor phase of liquid nitrogen and 37.0°C water bath (vortex mixing on high speed for 20 seconds between each freeze/ thaw), sonicating with 40% power for 30 seconds, and then a final vortex mixing on high speed for 30 seconds. Remove cell growth medium from the flask, and inoculate with a small volume of virus stock diluted in virus growth medium to provide a MOI of about 1-3. Adsorb 1-2 hours at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubate infected culture for 1-3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
Cell enlargement and clustering of rounded cells in cell culture; plaques with rounding and eventual sloughing.
Name of Depositor Dr. Zhiya Yu from Surgery Branch/ NCI/ NIH
Source
rVV-hgp100 was constructed in Dr. Nicolas P. Restifo's laboratory of Surgery Branch/ NCI/ NIH in the early 1990's.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation