Rickettsia asiatica ATCC ® VR-1593™

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Rickettsiaceae; Rickettsieae
Agent	Rickettsia asiatica
Strain	IO-1
Common Name	Rickettsia asiatica
Applications	No information is available about the possible pathogenicity of this organism for vertebrate hosts.
Positive for Mycoplasma	Yes
Biosafety Level	3
Isolation	Fukushima, Japan
Product Format	frozen
Storage Conditions	Store at -60°C or colder

Comments	Type strain for newly identified rickettsial species, isolated from an Ixodes ovatus tick nymph in Fukushima prefecture, Japan, in 1993. The preparation available from ATCC may be contaminated with mycoplasma based on PCR results. No information is available about the possible pathogenicity of this organism for vertebrate hosts.
Effect on Host	Intracytoplasmic and intranuclear growth visible following staining with Gimenez stain. CPE in the form of cell rounding and sloughing of infected cells.

Growth Medium	ATCC^® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM) Fetal Bovine Serum
Recommended Host	Vero cells (ATCC CRL-1587)
Growth Conditions	Recommendations for Infection: 7 days at 32°C and 5% CO₂ in EMEM (ATCC 30-2003) with 2mM L-glutamine supplemented with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C

Effect on Host	Intracytoplasmic and intranuclear growth visible following staining with Gimenez stain. CPE in the form of cell rounding and sloughing of infected cells.

Name of Depositor	P Fournier
Special Collection	ATCC
Source	Isolated from an Ixodes ovatus nymph in Oozaso, Fukushima prefecture, Japan, 1993
References	Fujita H., et al. Rickettsia asiatica sp. nov., isolated in Japan. Int. J. Syst. Evol. Microbiol. 56: 2365-8, 2006. PubMed: 17012563 Vestris, G. et. al. Seven years' experience of isolation of Rickettsia spp. from clinical specimens using the shell vial cell culture assay. Ann N Y Acad Sci. 2003 Jun;990:371-74. PubMed: 12860657 Fujita, H. et.al., List of all isolates of spotted fever group rickettsiae from ticks in Japan 1993-1998. Ann Rep Ohara Hosp 42, 45-50

Permits

These permits may be required for shipping this product:

Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation

Rickettsia asiatica (ATCC® VR-1593™)