JC polyomavirus (ATCC® VR-1583)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen 1 mL per vial

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Classification Polyomaviridae, Polyomavirus
Deposited As JC virus
Agent JC polyomavirus
Strain MAD-4
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Derived by adaptation from SVG p12 cells (ATCC® CRL-8621™) product ATCC® VR-1397™ to COS-7 cells (ATCC® CRL-1651™). ATCC® VR-1397™ and ATCC® VR-1583™ have not been compared for sequence or infectivity in tissue cultures. This strain is highly neurooncogenic and differs from MAD-1 strain, in part by a 19 bp deletion that eliminates the TATA box. JC polyomavirus hemagglutinates human type O red blood cells (HA requires addition of 0.25% sodium deoxycholate).

Effect on Host
No CPE, viral proteins detectable by antibody
Recommended Host
COS-7 (ATCC® CRL-1651™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Freeze-thaw viral material 6 times prior to inoculation. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 7 days
Effect on Host
No CPE, viral proteins detectable by antibody
Name of Depositor EO Major, National Institute of Neurological Disorders and Stroke; ATCC®
Source Brain tumor of owl monkey
References

Frye S, et al. Efficient production of JC virus in SVG cells and the use of purified viral antigens for analysis of specific humoral and cellular immune response.. J. Virol. Methods 63: 81-92, 1997. PubMed: 9015278

Major EO, et al. Owl monkey astrocytoma cells in culture spontaneously produce infectious JC virus which demonstrates altered biological properties. J. Virol. 61: 1435-1441, 1987. PubMed: 3033272

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation