Human adenovirus 4 (ATCC® VR-1572)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus E  /  Product Format: frozen 1 mL per vial

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Classification Adenoviridae, Mastadenovirus, Human adenovirus E
Agent Human adenovirus 4
Strain RI-67
Respiratory research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived by adaptation from HEK cell product ATCC® VR-1081™ to HeLa-229 cells (ATCC® CCL-2.1™). ATCC® VR-1081™ and ATCC® VR-1572™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.
Effect on Host CPE, cell rounding and degeneration
Recommended Host HeLa-229 (ATCC® CCL-2.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days
Effect on Host CPE, cell rounding and degeneration
Name of Depositor WP Rowe, National Institute of Allergy and Infectious Diseases; ATCC®
Special Collection ATCC
Source Throat washings of patient, Fort Leonard Wood, Missouri, 1952-1953

Huebner RJ, et al. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N. Engl. J. Med. 251: 1077-1086, 1954. PubMed: 13223958

Werner JH, Hilleman MR. Recovery of new agent from patients with acute respiratory illness. Proc. Soc. Exp. Biol. Med. 85: 183-188, 1954. PubMed: 13134329

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation