Tacaribe virus (ATCC® VR-1556)

Classification: Arenaviridae, Arenavirus, New World Arenaviruses  /  Product Format: frozen

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Classification Arenaviridae, Arenavirus, New World Arenaviruses
Agent Tacaribe virus
Strain TRVL 11573
Common Name Tacaribe virus
Biosafety Level 2
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Effect on Host
Yes, in vitro effects: Cytopathic effects, cell rounding followed by degeneration of the rounding cells
CPE, rounding of cells
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host: Vero cells (ATCC CCL-81)

Growth Conditions
Temperature: 37.0°C
Duration: 2-8 days; 5% CO2 in air atmosphere is recommended; For best results cells should be 24 to 48 hours old and 70–80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: Cytopathic effects, cell rounding followed by degeneration of the rounding cells
CPE, rounding of cells
Name of Depositor ATCC, WG Downs
Special Collection NCRR Contract
Source
derived from existing strain
References

Downs WG. . Am. J. Trop. Med. Hyg. 12: 640, 1963.

i. c. inoculation

i. c. or i. p. inoculation

or colder

This is tissue culture adapted from mouse preparation ATCC VR-114 which was isolated in 1956, Port of Spain, British West Indies, from brain of bat [Artibeus literatus palmarum].

y. s. inoculation

Can be passed serially (i.c.) in adult mice, animals become ill in 5-7 days and die within 24 hours with evidence of CNS involvement. The natural clinical illness in bats resembles that produced by rabies virus. Antigenic relationship to Junin virus of Argentinian and to Machupo virus of Bolivian hemorrhagic fevers has been demonstrated by means of the CF test.

or colder

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation