Vero cells (ATCC CCL-81)
Recommended Host: Vero cells (ATCC® CCL-81)
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI of about 0.1. Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding VGM.
Incubation: Incubate infected Vero cell culture for 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. Incubate for 3 days on CAM.