Orf virus (ATCC® VR-1548)

Classification: Poxviridae, Chordopoxvirinae, Parapoxvirus  /  Product Format: frozen

Permits and Restrictions

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Classification Poxviridae, Chordopoxvirinae, Parapoxvirus
Agent Orf virus
Strain NZ 2
Positive for Mycoplasma Yes
Biosafety Level 2
Isolation
Dunedin New Zealand
Product Format frozen
Storage Conditions -70°C or colder
Effect on Host
Yes, in vitro effects: Cytopathic effects - plaques of rounding cells that eventually slough off
CPE: plaques of rounding cells that eventually slough off
Recommended Host

Host of Choice: Sheep testes (ATCC CRL-6546) cells

Host Range: ovine and bovine testes cells

Growth Conditions
Temperature: 37.0°C
Duration: 3-4 days, rocking may be necessary to increase titer.
Effect on Host
Yes, in vitro effects: Cytopathic effects - plaques of rounding cells that eventually slough off
CPE: plaques of rounding cells that eventually slough off
Name of Depositor A Mercer
Special Collection Orthopoxvirus
Source
Dunedin New Zealand
Geographical Isolation Otago
References

contaminated with Mycoplasma orale

Housawi FM, et al. The reactivity of monoclonal antibodies against orf virus with other parapoxviruses and the identification of a 39 kDa immunodominant protein.. Arch. Virol. 143: 2289-2303, 1998. PubMed: 9930187

skin scab of an Otago, New Zealand sheep

Cross References

Nucleotide (GenBank) : OVU06671 orf virus NZ-2 putative virion envelope antigen (B2L) and (B3L) genes, complete cds.

Nucleotide (GenBank) : AF097215 Orf virus (strain NZ2) immunodominant envelope antigen F1L (F1L) gene, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation