Orf virus (ATCC^® VR-1548^™)

Orf virus ATCC^® VR-1548^™

frozen

For-Profit: $354.00 Non-Profit: $295.00

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Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Classification	Poxviridae, Chordopoxvirinae, Parapoxvirus
Agent	Orf virus
Strain	NZ 2
Positive for Mycoplasma	Yes
Biosafety Level	2
Isolation	Dunedin New Zealand
Product Format	frozen
Storage Conditions	-70°C or colder

Effect on Host	Yes, in vitro effects: Cytopathic effects - plaques of rounding cells that eventually slough off CPE: plaques of rounding cells that eventually slough off

Recommended Host	Host of Choice: Sheep testes (ATCC CRL-6546) cells Host Range: ovine and bovine testes cells
Growth Conditions	Temperature: 37.0°C Duration: 3-4 days, rocking may be necessary to increase titer.

Effect on Host	Yes, in vitro effects: Cytopathic effects - plaques of rounding cells that eventually slough off CPE: plaques of rounding cells that eventually slough off

Name of Depositor	A Mercer
Special Collection	Orthopoxvirus
Source	Dunedin New Zealand
Geographical Isolation	Otago
References	contaminated with Mycoplasma orale Housawi FM, et al. The reactivity of monoclonal antibodies against orf virus with other parapoxviruses and the identification of a 39 kDa immunodominant protein.. Arch. Virol. 143: 2289-2303, 1998. PubMed: 9930187 skin scab of an Otago, New Zealand sheep
Cross References	Nucleotide (GenBank) OVU06671 orf virus NZ-2 putative virion envelope antigen (B2L) and (B3L) genes, complete cds. Nucleotide (GenBank) AF097215 Orf virus (strain NZ2) immunodominant envelope antigen F1L (F1L) gene, complete cds.

Permits

These permits may be required for shipping this product:

Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

Basic Documentation