Orf virus (ATCC® VR-1548)

Classification: Poxviridae, Chordopoxvirinae, Parapoxvirus  /  Product Format: frozen

Classification Poxviridae, Chordopoxvirinae, Parapoxvirus
Agent Orf virus
Strain NZ 2
Positive for Mycoplasma Yes
Biosafety Level 2
Isolation
Dunedin New Zealand
Product Format frozen
Storage Conditions -70°C or colder
Effect on Host
Yes, in vitro effects: Cytopathic effects - plaques of rounding cells that eventually slough off
CPE: plaques of rounding cells that eventually slough off
Recommended Host

Host of Choice: Sheep testes (ATCC CRL-6546) cells

Host Range: ovine and bovine testes cells

Growth Conditions
Temperature: 37.0°C
Duration: 3-4 days, rocking may be necessary to increase titer.
Effect on Host
Yes, in vitro effects: Cytopathic effects - plaques of rounding cells that eventually slough off
CPE: plaques of rounding cells that eventually slough off
Name of Depositor A Mercer
Special Collection Orthopoxvirus
Source
Dunedin New Zealand
Geographical Isolation Otago
References

contaminated with Mycoplasma orale

Housawi FM, et al. The reactivity of monoclonal antibodies against orf virus with other parapoxviruses and the identification of a 39 kDa immunodominant protein.. Arch. Virol. 143: 2289-2303, 1998. PubMed: 9930187

skin scab of an Otago, New Zealand sheep

Cross References

Nucleotide (GenBank) : OVU06671 orf virus NZ-2 putative virion envelope antigen (B2L) and (B3L) genes, complete cds.

Nucleotide (GenBank) : AF097215 Orf virus (strain NZ2) immunodominant envelope antigen F1L (F1L) gene, complete cds.