Adeno-associated virus 5 (ATCC® VR-1523)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus 5  /  Product Format: frozen

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Classification Parvoviridae, Dependovirus, Adeno-associated virus 5
Agent Adeno-associated virus 5
Strain Original
Applications
Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
clinical specimen - human
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Depends upon adenovirus for replication. Suggest VR-846, as helper virus, which produces CPE on ATCC CCL-7.1 cells. *Helper virus may be inactivated by heating to 56C for 30 min.
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  LLC-MK2 (ATCC® CCL-7.1TM)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 4 - 5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). [Note: requires viral helper for productive infections, e.g., VR-846]
Cryopreservation
Storage temperature: -70°C
Effect on Host
in vitro effects: Adeno-associated virus shows no independent cytopathic effects
Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE.
Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing
Name of Depositor U Bantel-Schaal, H zur Hausen, U Bantel-Schaal
Special Collection NCRR Contract
Source
clinical specimen - human
References

or colder

Georg-Fries B, et al. Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. Virology 134: 64-71, 1984. PubMed: 6200995

Bantel-Schaal U, zur Hausen H. Characterization of the DNA of a defective human parvovirus isolated from a genital site. Virology 134: 52-63, 1984. PubMed: 6324476

can also be grown on other human and rodent cell lines

penile flat condylomatous lesion

requires viral helper for productive infections

confirm growth with PCR

Bantel-Schaal U, et al. Human adeno-associated virus type 5 is only distantly related to other known primate helper-dependent parvoviruses. J. Virol. 73: 939-947, 1999. PubMed: 9882294

Bantel-Schaal U, et al. Endocytosis of adeno-associated virus type 5 leads to accumulation of virus particles in the Golgi compartment. J. Virol. 76: 2340-2349, 2002. PubMed: 11836412

or colder

Cross References

Nucleotide (GenBank) : AF085716 Adeno-associated virus 5 DNA binding trs helicase (Rep22) and capsid protein (VP1) genes, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation