Vaccinia virus (ATCC® VR-1441)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Deposited As Vaccinia virus, Orthopoxvirus
Agent Vaccinia virus
Strain IHD-W
Applications
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s.
The host range includes most mammalian cells and tissues.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no.
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s.
Biosafety Level 2
Isolation
The IHD-W subtype was derived in Japan from IHD-J strain of vaccinia by selection for the ability to induce distinct clear plaques in culture.
Product Format frozen
Storage Conditions -70°C
Comments
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from the IHD-J parent strain, in that IHD-W is unable to induce an active hemagglutinin in the plasma membrane and does elicit polykaryoctosis.
The host range includes most mammalian cells and tissues.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from IHD-J in that IHD-W is HA(-) and IHD-J is HA(+).
-70C
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Recommended Host
L2 cells (ATCC CCL-149); chicken embryo fibroblasts

Host of Choice: L2 and CEF

Host Range: most mammalian cells and tissues

most mammalian cells and tissues
Growth Conditions
Duration: 1-5 days at 37C
Cryopreservation
Storage temperature: -70C
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Name of Depositor S Dales
Special Collection NCRR Contract
Source
The IHD-W subtype was derived in Japan from IHD-J strain of vaccinia by selection for the ability to induce distinct clear plaques in culture.
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807