Herpesvirus saimiri (ATCC® VR-1414)

Classification: Herpesviridae, Rhadinovirus  /  Product Format: frozen

Classification Herpesviridae, Rhadinovirus
Agent Herpesvirus saimiri
Strain C-488 (US)
Common Name Herpesvirus saimiri
Biosafety Level 2
Isolation
derived from ATCC VR-1396, which came from the blood lymphocytes of a squirrel monkey (Saimiri sciureus) in New England
Product Format frozen
Storage Conditions -70°C or colder
Comments
VR-1414 (C-488 US) is derived fron VR-1396 and is known to differ only in passage history and permit requirements. Both strains transform human T lymphocytes to antigen- and mitogen-independent, but IL-2 dependent for stable growth.
For methods of preparation see: Fickenscher H. and B. Fleckenstein, 1994. in: Methods in Molecular Genetics. (Ed.) K. W. Adolph, 4: 345-362. Academic Press San Diego.
-70C or lower
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of detached cells bordered by rounded enlarged cells)
CPE (foci of detached cells bordered by        rounded enlarged cells)
Recommended Host
OMK cells (ATCC CRL-1556)

Host of Choice: OMK cells (ATCC CRL-1556)

Host Range: OMK and many other cell lines

OMK cells (ATCC CRL-1556)
Growth Conditions
Duration: 10-20 days at 37C in OMK cells (ATCC CRL-1556)
Cryopreservation
Storage temperature: -70C or lower
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of detached cells bordered by rounded enlarged cells)
CPE (foci of detached cells bordered by        rounded enlarged cells)
Name of Depositor H Fickenscher
Special Collection NCRR Contract
Source
derived from ATCC VR-1396, which came from the blood lymphocytes of a squirrel monkey (Saimiri sciureus) in New England
References

Biesinger B, et al. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology 176: 505-514, 1990. PubMed: 2161148

Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J. Virol. 43: 352-356, 1982. PubMed: 6287011