Herpesvirus saimiri (ATCC® VR-1396)

Classification: Herpesviridae, Rhadinovirus  /  Product Format: frozen

Permits and Restrictions

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Classification Herpesviridae, Rhadinovirus
Agent Herpesvirus saimiri
Strain C-488
Common Name Herpesvirus saimiri
Biosafety Level 2
Isolation
Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus)
Product Format frozen
Storage Conditions  -70°C or colder
Comments
This item is also available as VR-1414 (herpesvirus saimiri), which does not require a USDA permit.
Herpesvirus saimiri strain C488 transforms human T lymphocytes to antigen- and mitogen-independent, but IL-2 dependent stable growth. Virus stocks are stable for months at 4C, but titer greatly reduced by freeze-thaw cycles.
For methods of propagation see: Fickenscher and Fleckenstein, 1994. in: Methods in Molecular Genetics. (ed) K. W. Adolph, 4: 345-362, 1994.
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of detached cells boardered by rounded enlarged cells)
 CPE, foci of detached cells bordered by rounded enlarged cells
Recommended Host
OMK cells (ATCC CRL-1556)

Production Host:  OMK (ATCC® CRL-1556)

Growth Conditions
Temperature: 37.0°C
Duration: 10-20 days
Effect on Host
Yes, in vitro effects: Cytopathic effects (foci of detached cells boardered by rounded enlarged cells)
 CPE, foci of detached cells bordered by rounded enlarged cells
Name of Depositor M Daniel, R Desrosiers, B Biesinger, B Fleckenstein, H Fickenscher
Special Collection NCRR Contract
Source
Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus)
References

Biesinger B, et al. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology 176: 505-514, 1990. PubMed: 2161148

Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J. Virol. 43: 352-356, 1982. PubMed: 6287011

Biesinger B, et al. Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proc. Natl. Acad. Sci. USA 89: 3116-3119, 1992. PubMed: 1313581

. Methods in Molecular Genetics, Part A. : K.W. Adolph; 1993.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation