Ectromelia virus ATCC ® VR-1374™

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification	Poxviridae, Orthopoxvirus
Agent	Ectromelia virus
Strain	Moscow
Applications	1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use.
Biosafety Level	2
Isolation	tissue, animal
Product Format	frozen
Storage Conditions	-70°C or colder (or colder)

Comments	Inadvertent introduction of Ectromelia virus into mouse colonies has caused major outbreaks in US animal colonies. The depositor recommends that animal care and biosafety management at your institution be consulted prior to initiating work with this agent. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use. For additional information see: Fenner. 1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. II. Diseases, (ed) Foster et al., Academic Press.
Effect on Host	Yes, in vitro effects: cytopathic effects, refractile rounding CPE, refractile rounding; death in mice Yes, in vivo effects: death of mouse

Comments

Inadvertent introduction of Ectromelia virus into mouse colonies has caused major outbreaks in US animal colonies. The depositor recommends that animal care and biosafety management at your institution be consulted prior to initiating work with this agent. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use. For additional information see: Fenner. 1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. II. Diseases, (ed) Foster et al., Academic Press.

Effect on Host

Yes, in vitro effects: cytopathic effects, refractile rounding

CPE, refractile rounding; death in mice

Yes, in vivo effects: death of mouse

Growth Medium	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host	Mus musculus mouse Recommended Host: BS-C-1 (ATCC^® CCL-26^TM)
Growth Conditions	Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 1-7 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Cryopreservation	Storage temperature: -70°C

Effect on Host	Yes, in vitro effects: cytopathic effects, refractile rounding CPE, refractile rounding; death in mice Yes, in vivo effects: death of mouse

Name of Depositor	RM Buller
Special Collection	ATCC
Source	tissue, animal
References	Chen W, et al. Restricted replication of ectromelia virus in cell culture correlates with mutations in virus-encoded host range gene. Virology 187: 433-442, 1992. PubMed: 1546448 or colder spleen homogenate from a BALB/c mouse 6 days post-infection or colder
Cross References	Nucleotide (GenBank) AF012825 Ectromelia virus envelope glycoprotein C1R (C1R), C2R (C2R), C3R (C3R), IFN-gamma binding protein C4R (C4R), C5R (C5R), protein kinase C6R (C6R), serpin C7R (C7R), C8R (C8R), IL-1 beta binding protein C9R (C9R), C10L (C10L), C11R (C11R), IFN-...

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Ectromelia virus (ATCC® VR-1374™)

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Ectromelia virus (ATCC^® VR-1374^™)