Piscirickettsia salmonis Fryer et al. (ATCC® VR-1361)

Classification: Thiotrichales, Piscirickettsiaceae, Piscirickettsia  /  Product Format: frozen

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Classification Thiotrichales, Piscirickettsiaceae, Piscirickettsia
Agent Piscirickettsia salmonis Fryer et al.
Strain LF-89
Biosafety Level 2 This is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist.
Isolation
clinical specimen - animal
Chile
Isolation date: October, 1989
Product Format frozen
Storage Conditions -70°C or colder
Comments
This organism is a gram-negative, non-motile, pleomorphic 0.5 to 1.5 micometer diameter coccoid.
Effect on Host
Cytopathic effects in tissue culture
CPE; small refractile rounding and clumping
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10 mM HEPES, 90%; fetal bovine serum, 10%
Recommended Host

Recommended Host:  CHSE-214 (ATCC® CRL-1681TM)

Growth Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 15°C to 18°C
Duration: 6 to 14 days; For best results host cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Effect on Host
Cytopathic effects in tissue culture
CPE; small refractile rounding and clumping
Name of Depositor JL Fryer, CN Lannan
Special Collection ATCC
Source
clinical specimen - animal
Chile
Isolation date: October, 1989
Geographical Isolation South America
Year of Origin October, 1989
References

Fryer JL, Mauel MJ. The rickettsia: an emerging group of pathogens in fish. Emerg. Infect. Dis. 3: 137-144, 1997. PubMed: 9204294

Fryer JL, et al. Isolation of a rickettsiales-like organism from diseased Coho salmon (Oncorhynchus kisutch) in Chile. Fish Pathol. 25: 107-114, 1990.

VR-1361 is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist.

Fryer JL, et al. Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes. Int. J. Syst. Bacteriol. 42: 120-126, 1992. PubMed: 1371057

Isolated by J. L. Fryer and C. N. Lannan from the kidney of a Coho salmon (Oncorhynchus kisutch) displaying lethargy, anemia, swollen kidney, splenomegaly, darkened coloration and rare hepatic lesions.

small refractile rounding and clumping

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