Hepatitis A virus (ATCC® VR-1357)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen

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Classification Picornaviridae, Hepatovirus
Agent Hepatitis A virus
Strain PA21
Applications
Hepatitis A antigen can be detected in infected cells by IF and in infected cell culture supernate by ELISA. This item has fungal contamination that can, with difficulty, be controlled with Amphotericin B.
Enteric Research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Comments
PA21 is one of the better characterized primate HAV strains that differs significantly from the HM175-like isolates [Brown et al., J, Virol. 63: 4932-37, 1989]. Hepatitis A antigen can be detected in infected cells by IF and in infected cell culture supernate by ELISA. This item has fungal contamination that can, with difficulty, be controlled with Amphotericin B.
Effect on Host
No CPE, detect by IF, ELISA or PCR
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 2.5 ug/ml Amphotericin B Solution and 2% fetal bovine serum
Recommended Host
BSC-1 (ATCC® CCL-26™)
Growth Conditions

Temperature: 35°C

Incubation: 20 to 28 days at 35°C, a 5% CO2 in air atmosphere is recommended.

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).

Effect on Host
No CPE, detect by IF, ELISA or PCR
Name of Depositor LN Binn
Special Collection ATCC
Source From liver tissue of a naturally infected owl monkey in Panama
References

Binn LN, et al. Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures. J. Clin. Microbiol. 20: 28-33, 1984. PubMed: 6086708

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation