Vaccinia virus (ATCC® VR-1354)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

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Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus
Strain WR (NIH TC-adapted)
Applications
testing disinfectants
Biosafety Level 2
Isolation
multiple laboratory passages in cell culture of WR strain of vaccinia
Product Format frozen
Storage Conditions  -70°C or colder
Comments
This strain is recommended by ATCC for use in the tests described in Fed. Spec. A-A-1438 where only the taxon is specified.
The host range includes many mammalian and avian cells, e.g.,HeLa S3 (ATCC CCL-2.2) and BS-C-1 (ATCC CCL-26).
This tissue culture adapted WR strain of vaccinia is derived from ATCC VR-119 and has been shown to serve as an effective expression vector for foreign genes (Mackett et al., J. Virol., 1984)
Dr. Moss received the WR strain in 1966 from Dr. Salzman who obtained the virus as VR-119 from the ATCC. The tissue culture adapted strain WR has been widely distributed and used in constructing vectors for gene expression (J. Gen Virol, 1986).
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).
Cell associated virus from infected HeLa cells
Effect on Host
Yes, in vitro effects: Cytopathic effects (localized enlarged, rounded cells which eventually extend throughout the monolayer)
 CPE, cell rounding and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host
Vero (ATCC CCL-81)
Growth Conditions
Temperature: 37°C
Incubation: 2-3 days at 37°C
Effect on Host
Yes, in vitro effects: Cytopathic effects (localized enlarged, rounded cells which eventually extend throughout the monolayer)
 CPE, cell rounding and sloughing
Name of Depositor NS Cooper, B Moss
Special Collection ATCC
Source
multiple laboratory passages in cell culture of WR strain of vaccinia
References

Salzman NP, Sebring ED. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J. Virol. 1: 16-23, 1967. PubMed: 4248263

Mackett M, et al. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc. Natl. Acad. Sci. USA 79: 7415-7419, 1982. PubMed: 6296831

Cross References

Nucleotide (GenBank) : S64006 E3L=double-stranded RNA-binding protein [vaccinia virus, WR, ATCC VR1354, Genomic, 633 nt].

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation