Minute virus of mice (ATCC® VR-1346)

Classification: Parvoviridae, Parvovirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Parvoviridae, Parvovirus
Agent Minute virus of mice
Strain Prototype (p)
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, slight rounding and enlarging
Recommended Host
A9 (ATCC® CCL-1.4™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 60-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Incubation: 2-3 days
Effect on Host
CPE, slight rounding and enlarging
Name of Depositor P Tattersall, Yale University School of Medicine
Source Plaque-purified from Minute virus of mice, strain Crawford
References

Tattersall P, Bratton J. Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. J. Virol. 46: 944-955, 1983. PubMed: 6602222

Tattersall P. Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth. J. Virol. 10: 586-590, 1972. PubMed: 4673484

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation