Minute virus of mice (ATCC® VR-1346)

Classification: Parvoviridae, Parvovirus  /  Product Format: frozen 1 mL per vial

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Classification Parvoviridae, Parvovirus
Agent Minute virus of mice
Strain Prototype (p)
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, slight rounding and enlarging
Recommended Host
A9 (ATCC® CCL-1.4™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 60-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Incubation: 2-3 days
Effect on Host
CPE, slight rounding and enlarging
Name of Depositor P Tattersall, Yale University School of Medicine
Source Plaque-purified from Minute virus of mice, strain Crawford
References

Tattersall P, Bratton J. Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. J. Virol. 46: 944-955, 1983. PubMed: 6602222

Tattersall P. Replication of the parvovirus MVM. I. Dependence of virus multiplication and plaque formation on cell growth. J. Virol. 10: 586-590, 1972. PubMed: 4673484

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation