Encephalomyocarditis (Murine) (tissue culture adapted) (ATCC® VR-129B)

Classification: Picornavirus, Cardiovirus  /  Product Format: frozen 1 mL per vial

Classification Picornavirus, Cardiovirus
Agent Encephalomyocarditis (Murine) (tissue culture adapted)
Strain EMC
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-129B Micrograph
Comments

This strain is indistinguishable antigenically from strain MM and Mengo encephalomyocarditis virus.

Effect on Host
CPE, rounding and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor W Brandt
Source Chimpanzee, Florida, 1944
Year of Origin 1944
References

Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945.