Encephalomyocarditis virus (Murine) (tissue culture adapted) (ATCC® VR-129B)

Classification: Picornavirus, Cardiovirus  /  Product Format: frozen 1 mL per vial

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Classification Picornavirus, Cardiovirus
Deposited As Encephalomyocarditis (Murine) (tissue culture adapted)
Agent Encephalomyocarditis virus (Murine) (tissue culture adapted)
Strain EMC
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-129B Micrograph
Comments

This strain is indistinguishable antigenically from strain MM and Mengo encephalomyocarditis virus.

Effect on Host
CPE, rounding and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Incubation: 2-4 days
Effect on Host
CPE, rounding and sloughing
Name of Depositor W Brandt
Source Chimpanzee, Florida, 1944
Year of Origin 1944
References

Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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