BR1S-218.1 [PTA-4307] (ATCC® CRL-13025™) Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte; somatic cell hybri General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Cell Type hybridoma: B lymphocyte; somatic cell hybri Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 Applications These antibodies can be used to detect BRCA1 mutations.Animals were immunized with against a synthetic peptide containing BRCA1 amino acids 1840 to 1862. Spleen cells were fused with the mouse non-secretory myeloma cell line P3.653. Monoclonal antibodies BR1S-218.1 (ATCC PTA-4307), BR1S-060.2 (ATCC PTA-4306), BR1S-384.5 (ATCC PTA-4308), and BR1S-425.1 (ATCC PTA-4309) are against the C-terminal region of a full-length BRCA1 polypeptide (amino acids 1840-1862). Storage Conditions liquid nitrogen vapor phase Derivation Animals were immunized with against a synthetic peptide containing BRCA1 amino acids 1840 to 1862. Spleen cells were fused with the mouse non-secretory myeloma cell line P3.653. Monoclonal antibodies BR1S-218.1 (ATCC PTA-4307), BR1S-060.2 (ATCC PTA-4306), BR1S-384.5 (ATCC PTA-4308), and BR1S-425.1 (ATCC PTA-4309) are against the C-terminal region of a full-length BRCA1 polypeptide (amino acids 1840-1862). Monoclonal antibodies BR1N.129.5 (ATCC PTA-4304) and BR1N-411.4 (ATCC PTA-4305) are against the N-terminal region of a BRCA1 polypeptide (amino acids 1 to 304). Monoclonal antibodies BR1H-788.6 (ATCC PTA-4301), BR1H-945.2 (ATCC PTA-4303) and BR1H-826.5 (ATCC PTA-4302) are against a portion of a BRCA1 polypeptide between the N-terminal and C-terminal regions of the BRCA1 polypeptide (amino acids 1360 to 1555). These antibodies can be used to detect BRCA1 mutations. Comments Animals were immunized with against a synthetic peptide containing BRCA1 amino acids 1840 to 1862. Spleen cells were fused with the mouse non-secretory myeloma cell line P3.653. Monoclonal antibodies BR1S-218.1 (ATCC PTA-4307), BR1S-060.2 (ATCC PTA-4306), BR1S-384.5 (ATCC PTA-4308), and BR1S-425.1 (ATCC PTA-4309) are against the C-terminal region of a full-length BRCA1 polypeptide (amino acids 1840-1862). Monoclonal antibodies BR1N.129.5 (ATCC PTA-4304) and BR1N-411.4 (ATCC PTA-4305) are against the N-terminal region of a BRCA1 polypeptide (amino acids 1 to 304). Monoclonal antibodies BR1H-788.6 (ATCC PTA-4301), BR1H-945.2 (ATCC PTA-4303) and BR1H-826.5 (ATCC PTA-4302) are against a portion of a BRCA1 polypeptide between the N-terminal and C-terminal regions of the BRCA1 polypeptide (amino acids 1360 to 1555). These antibodies can be used to detect BRCA1 mutations. Complete Growth Medium HH4 Medium supplemented with 10% fetal bovine serum Cryopreservation Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37.0°C Isotype mouse IgG1 Name of Depositor Hybritech, Inc. References Knott CL, Kuus-Reichel K. Methods of detecting BRCA1 mutations. U.S. Patent 6,514,713 dated Feb 4 2003 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Knott CL, Kuus-Reichel K. Methods of detecting BRCA1 mutations. U.S. Patent 6,514,713 dated Feb 4 2003