Stem Cell Culture Reagents and Media

  • Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadipocytes (ATCC® PCS-500-050)

    ATCC® Number: PCS-500-050
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications:

    The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:
    1. One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation
    2. One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation
    3. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

    *The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format. 



    For-Profit:   $162.00 Non-Profit:   $162.00

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  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020
    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $51.00 Non-Profit:   $51.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs (ATCC® PCS-500-053)

    ATCC® Number: PCS-500-053
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications: The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs contains medium and reagents designed to induce adipogenesis in actively proliferating Bone Marrow and Umbilical-Derived Mesenchymal Stem Cells with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components: 

    1. One vial containing 10 mL of ADM Supplement BMMSC/UCMSC for use in initiation of adipocyte differentiation 
    2. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation 

    * The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format.

    For-Profit:   $162.00 Non-Profit:   $162.00

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  • For-Profit:   $142.00 Non-Profit:   $142.00

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  • Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC® PCS-500-030)

    ATCC® Number: PCS-500-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This low serum (2% FBS) system is designed to support mesenchymal stem cells derived from various normal human tissues including lipoaspirates and umbilical cord. Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of adult stem cells, Mesenchymal Stem Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of undifferentiated mesenchymal stem cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

    1. For mesenchymal stem cells derived from human lipoaspirates (e.g., Adipose-Derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-011) or umbilical cord (Umbilical Cord-Derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-010), supplement Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs with the Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs –Low serum (ATCC PCS-500-040).
    2. For mesenchymal stem cells derived from bone marrow (Bone Marrow-derived Mesenchymal Stem Cells; Normal, Human, ATCC PCS-500-012) supplement Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs with the Mesenchymal  Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC PCS-500-041)
    3. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $61.00 Non-Profit:   $61.00

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    Product Format: 485 mL
    Comments: