MEF (C57BL/6) IRR (ATCC® SCRC-1008.1)

Organism: Mus musculus, mouse  /  Cell Type: Fibroblast  /  Tissue: Embryo, whole  / 

Organism Mus musculus, mouse
Tissue Embryo, whole
Cell Type Fibroblast
Product Format frozen
Morphology Fibroblast
Culture Properties Adherent
Biosafety Level 1
Age 14 days gestation embryo
Gender Male and female mixed
Strain C57BL/6
Applications
These cells are provided for use as feeder cells to support the growth of stem cells in the undifferentiated state.
Storage Conditions Liquid nitrogen vapor phase
Clinical Data
male and female mixed
Comments

These cells have been growth-arrested by irradiation with 10,000 rads.  The cells will begin to deteriorate in 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days.

Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 4 to 5 X 106 cells per T75 flask in order to obtain a supportive monolayer for stem cell growth.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing

    Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported.   It is recommended that the feeder cells be plated 24 hours before use at 6 X 10cells/cm2 in order to obtain a supportive monolayer for stem cell growth.

    Medium Renewal: Twice a week or when pH decreases

    Cryopreservation
    Liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor ATCC
    References

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    Basic Documentation
    References

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.