Perkinsus honshuensis (ATCC® PRA-176)

Organism: Perkinsus honshuensis  /  Depositor: C Dungan

Strain Designations Mie-3G
Biosafety Level 2
Isolation
marine invertebrate
Mie, Japan
Isolation date: May 29, 2002
Product Format frozen
Type Strain yes
Comments
This species was isolated from gill tissue from the Manila Clam, Venerupis ( = Tapes, Ruditapes) philippinarum. This strain is the hapantotype representative of Perkinsus honshuensis.
Medium ATCC® Medium 1886: Perkinsus broth medium
Growth Conditions
Temperature: 25.0°C
Max Temperature: 28.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Subcultivation
Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Name of Depositor C Dungan
Special Collection NSF - Protistology
Geographical Isolation Gokasho Bay
Year of Origin May 29, 2002
References

. Synonymy of Perkinsus olseni Lester & Davis, 1981 and P. atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus . Bull. Eur. Ass. Fish Pathol. 22: 258-265, 2002.

Dungan C F, Reece K S. In vitro propagation of two Perkinsus spp. parasites from japanese manila clams Venerupis philippinarum, and description of Perkinsus honshuensis n. sp.. J. Eukaryot. Microbiol. in press: 2006..

Cross References

Nucleotide (GenBank) : DQ516697 ITS rDNA

Nucleotide (GenBank) : DQ516698 ITS rDNA

Nucleotide (GenBank) : DQ516696 ITS rDNA