Skip to Content

Sign In
|
Create a Profile
|
Quick Order
|
Shopping Cart (0 items)
|
USA

Select another country:

Home
Products
PRA-113 ^™

Acanthamoeba hatchetti Sawyer et al. (ATCC^® PRA-113^™)

Organism: Acanthamoeba hatchetti Sawyer et al. / Depositor: J Walochnik

Acanthamoeba hatchetti Sawyer et al. ATCC^® PRA-113^™

frozen

For-Profit: $354.00 Non-Profit: $295.00

Qty:

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	2HH
Biosafety Level	2
Isolation	clinical specimen - human Vienna Austria Isolation date: 1996
Product Format	frozen
Storage Conditions	liquid nitrogen vapor phase
Type Strain	no

Medium	ATCC^® Medium 712: PYG w/ Additives
Growth Conditions	Temperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain. Interval: every month
Subcultivation	Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain. Interval: every month
Cryopreservation	Storage temperature: liquid nitrogen vapor phase

Name of Depositor	J Walochnik
Chain of Custody	J Walochnik
Year of Origin	1996
References	Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936 cornea of patient with keratitis Hiti K, et al. Microwave treatment of contact lens cases contaminated with acanthamoeba. Cornea 20: 467-470, 2001. PubMed: 11413399

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS