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PRA-106 ^™

Acanthamoeba castellanii (Douglas) Page (ATCC^® PRA-106^™)

Organism: Acanthamoeba castellanii (Douglas) Page / Depositor: J Walochnik

Acanthamoeba castellanii (Douglas) Page ATCC^® PRA-106^™

frozen

For-Profit: $354.00 Non-Profit: $295.00

Qty:

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	40AB
Biosafety Level	2
Isolation	clinical specimen - animal Vienna Austria Isolation date: 1998
Product Format	frozen
Storage Conditions	liquid nitrogen vapor phase
Type Strain	no

Medium	ATCC^® Medium 712: PYG w/ Additives
Growth Conditions	Temperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month
Subcultivation	Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month
Cryopreservation	Storage temperature: liquid nitrogen vapor phase

Name of Depositor	J Walochnik
Chain of Custody	J Walochnik
Year of Origin	1998
References	Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 necrotic tissue of a Basiliscus plumifrons (green basilisk) Walochnik J, et al. Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basilliscus plumifrons (Sauria: Iguanidae). Parasitol. Res. 85: 601, 1999. PubMed: 10382612

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS