Herpetomonas roitmani (Fiorini et al.) Faria-e-Silva et al. (ATCC® 50305)

Organism: Herpetomonas roitmani (Fiorini et al.) Faria-e-Silva et al.  /  Depositor: MJ Soares

Biosafety Level 1
Isolation
Ornidia obesa (Diptera: Syrphidae), Brazil, 1988
Product Format frozen
Type Strain no
Comments
species description
Medium ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor MJ Soares
Year of Origin 1988
References

Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148

Fiorini JE, et al. Tres novas especies de tripanosomatideos de insetos isolados em alfenas, minas gerais, Basil. Mem. Inst. Oswaldo Cruz Rio J. 84: 69-74, 1989.