Monocercomonas sp. ATCC ® 50210™

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	Ns-1PRR
Application	Phylogeny based upon superoxide dismutase gene sequence analysis
Biosafety Level	1
Isolation	water snake, Natrix sipedon, Laurel, MD, 1959
Product Format	frozen
Type Strain	no

Comments	Phylogeny based upon superoxide dismutase gene sequence analysis AF005077, partial eIF-2 gamma gene sequence phylogeny New Monocercomonas from the water snake Tubulin subunits and phylogeny

Medium	LYI Entamoeba medium Casein Digest (BBL 97023), 20.0 g Yeast Extract (BBL 11928), 10.0 g NaCl, 2.0 g L-Cysteine . HCl (Sigma C7880), 1.0 g Ascorbic Acid (J.T. Baker B582-5), 0.2 g Ferric Ammonium Citrate (Mallinckrodt 0658), 22.8 mg Distilled water, 770.0 ml Adjust pH to 6.8 with NaOH. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following solutions: Solution A: Dextrose, 10.0 g Distilled water, 50.0 ml Filter-sterilize. Solution B: K2HPO4, 1.0 g KH2PO4, 0.6 g Distilled water, 50.0 ml Autoclave at 121C for 15 minutes. Solution C: Bovine serum (heat-inactivated), 100.0 ml Solution D: Special 107 Vitamin Mix (see below), 30.0 ml Special 107 Vitamin Mix: Aseptically combine the following: Solution 1 (see below), 0.4 ml Solution 2 (see below), 1.2 ml Solution 3 (see below), 0.4 ml Sterile distilled water, 18.0 ml Solution 4 Vitamins (see below), 100.0 ml Total volume of complete Special 107 Vitamin Mix is 120 ml. Solution 1 (filter-sterilized): D,L-6,8-Thioctic acid, oxidized (Sigma T1395), 100.0 mg Absolute Ethanol, 100.0 ml Solution 2 (filter-sterilized): Vitamin B12 (Sigma V2876), 40.0 mg Distilled water, 100.0 ml Solution 3 (filter-sterilized): Tween 80 (Sigma P1754), 50.0 g Absolute Ethanol, 100.0 ml Solution 4 Vitamins: alpha Tocopherol phosphate, disodium salt, 0.025 mg d-Biotin, 0.025 mg Calciferol (Vitamin D2), 0.250 mg Calcium D-(+)-pantothenate, 0.025 mg Choline chloride, 1.250 mg Folic acid, 0.025 mg i-Inositol, 0.125 mg Menadione (Vitamin K3), 0.025 mg Niacin, 0.0625 mg Niacinamide, 0.0625 mg p-Aminobenzoic acid, 0.125 mg Pyridoxal . HCl, 0.0625 mg Pyridoxine . HCl, 0.0625 mg Riboflavin, 0.025 mg Thiamine . HCl, 0.025 mg Vitamin A (crystallized alcohol), 0.250 mg Vitamin B12, 10.000 mg Distilled water, 1.0 L Some lots of Casein Digest, Yeast Extract, or serum may inhibit growth.7071 LYI Entamoeba medium Casein Digest (BBL 97023), 20.0 g Yeast Extract (BBL 11928), 10.0 g NaCl, 2.0 g L-Cysteine . HCl (Sigma C7880), 1.0 g Ascorbic Acid (J.T. Baker B582-5), 0.2 g Ferric Ammonium Citrate (Mallinckrodt 0658), 22.8 mg Distilled water, 770.0 ml Adjust pH to 6.8 with NaOH. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following solutions: Solution A: Dextrose, 10.0 g Distilled water, 50.0 ml Filter-sterilize. Solution B: K2HPO4, 1.0 g KH2PO4, 0.6 g Distilled water, 50.0 ml Autoclave at 121C for 15 minutes. Solution C: Bovine serum (heat-inactivated), 100.0 ml Solution D: Special 107 Vitamin Mix (see below), 30.0 ml Special 107 Vitamin Mix: Aseptically combine the following: Solution 1 (see below), 0.4 ml Solution 2 (see below), 1.2 ml Solution 3 (see below), 0.4 ml Sterile distilled water, 18.0 ml Solution 4 Vitamins (see below), 100.0 ml Total volume of complete Special 107 Vitamin Mix is 120 ml. Solution 1 (filter-sterilized): D,L-6,8-Thioctic acid, oxidized (Sigma T1395), 100.0 mg Absolute Ethanol, 100.0 ml Solution 2 (filter-sterilized): Vitamin B12 (Sigma V2876), 40.0 mg Distilled water, 100.0 ml Solution 3 (filter-sterilized): Tween 80 (Sigma P1754), 50.0 g Absolute Ethanol, 100.0 ml Solution 4 Vitamins: alpha Tocopherol phosphate, disodium salt, 0.025 mg d-Biotin, 0.025 mg Calciferol (Vitamin D2), 0.250 mg Calcium D-(+)-pantothenate, 0.025 mg Choline chloride, 1.250 mg Folic acid, 0.025 mg i-Inositol, 0.125 mg Menadione (Vitamin K3), 0.025 mg Niacin, 0.0625 mg Niacinamide, 0.0625 mg p-Aminobenzoic acid, 0.125 mg Pyridoxal . HCl, 0.0625 mg Pyridoxine . HCl, 0.0625 mg Riboflavin, 0.025 mg Thiamine . HCl, 0.025 mg Vitamin A (crystallized alcohol), 0.250 mg Vitamin B12, 10.000 mg Distilled water, 1.0 L Some lots of Casein Digest, Yeast Extract, or serum may inhibit growth.
Growth Conditions	Temperature: 25.0°C Duration: axenic; anaerobic

Medium

LYI Entamoeba medium Casein Digest (BBL 97023)*, 20.0 g Yeast Extract (BBL 11928)*, 10.0 g NaCl, 2.0 g L-Cysteine . HCl (Sigma C7880), 1.0 g Ascorbic Acid (J.T. Baker B582-5), 0.2 g Ferric Ammonium Citrate (Mallinckrodt 0658), 22.8 mg Distilled water, 770.0 ml Adjust pH to 6.8 with NaOH. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following solutions: Solution A: Dextrose, 10.0 g Distilled water, 50.0 ml Filter-sterilize. Solution B: K2HPO4, 1.0 g KH2PO4, 0.6 g Distilled water, 50.0 ml Autoclave at 121C for 15 minutes. Solution C: Bovine serum (heat-inactivated), 100.0 ml Solution D: Special 107 Vitamin Mix (see below), 30.0 ml Special 107 Vitamin Mix: Aseptically combine the following: Solution 1 (see below), 0.4 ml Solution 2 (see below), 1.2 ml Solution 3 (see below), 0.4 ml Sterile distilled water, 18.0 ml Solution 4 Vitamins (see below), 100.0 ml Total volume of complete Special 107 Vitamin Mix is 120 ml. Solution 1 (filter-sterilized): D,L-6,8-Thioctic acid, oxidized (Sigma T1395), 100.0 mg Absolute Ethanol, 100.0 ml Solution 2 (filter-sterilized): Vitamin B12 (Sigma V2876), 40.0 mg Distilled water, 100.0 ml Solution 3 (filter-sterilized): Tween 80 (Sigma P1754), 50.0 g Absolute Ethanol, 100.0 ml Solution 4 Vitamins: alpha Tocopherol phosphate, disodium salt, 0.025 mg d-Biotin, 0.025 mg Calciferol (Vitamin D2), 0.250 mg Calcium D-(+)-pantothenate, 0.025 mg Choline chloride, 1.250 mg Folic acid, 0.025 mg i-Inositol, 0.125 mg Menadione (Vitamin K3), 0.025 mg Niacin, 0.0625 mg Niacinamide, 0.0625 mg p-Aminobenzoic acid, 0.125 mg Pyridoxal . HCl, 0.0625 mg Pyridoxine . HCl, 0.0625 mg Riboflavin, 0.025 mg Thiamine . HCl, 0.025 mg Vitamin A (crystallized alcohol), 0.250 mg Vitamin B12, 10.000 mg Distilled water, 1.0 L *Some lots of Casein Digest, Yeast Extract, or serum may inhibit growth.7071 LYI Entamoeba medium Casein Digest (BBL 97023)*, 20.0 g Yeast Extract (BBL 11928)*, 10.0 g NaCl, 2.0 g L-Cysteine . HCl (Sigma C7880), 1.0 g Ascorbic Acid (J.T. Baker B582-5), 0.2 g Ferric Ammonium Citrate (Mallinckrodt 0658), 22.8 mg Distilled water, 770.0 ml Adjust pH to 6.8 with NaOH. Autoclave at 121C for 15 minutes. Cool to room temperature and aseptically add the following solutions: Solution A: Dextrose, 10.0 g Distilled water, 50.0 ml Filter-sterilize. Solution B: K2HPO4, 1.0 g KH2PO4, 0.6 g Distilled water, 50.0 ml Autoclave at 121C for 15 minutes. Solution C: Bovine serum (heat-inactivated), 100.0 ml Solution D: Special 107 Vitamin Mix (see below), 30.0 ml Special 107 Vitamin Mix: Aseptically combine the following: Solution 1 (see below), 0.4 ml Solution 2 (see below), 1.2 ml Solution 3 (see below), 0.4 ml Sterile distilled water, 18.0 ml Solution 4 Vitamins (see below), 100.0 ml Total volume of complete Special 107 Vitamin Mix is 120 ml. Solution 1 (filter-sterilized): D,L-6,8-Thioctic acid, oxidized (Sigma T1395), 100.0 mg Absolute Ethanol, 100.0 ml Solution 2 (filter-sterilized): Vitamin B12 (Sigma V2876), 40.0 mg Distilled water, 100.0 ml Solution 3 (filter-sterilized): Tween 80 (Sigma P1754), 50.0 g Absolute Ethanol, 100.0 ml Solution 4 Vitamins: alpha Tocopherol phosphate, disodium salt, 0.025 mg d-Biotin, 0.025 mg Calciferol (Vitamin D2), 0.250 mg Calcium D-(+)-pantothenate, 0.025 mg Choline chloride, 1.250 mg Folic acid, 0.025 mg i-Inositol, 0.125 mg Menadione (Vitamin K3), 0.025 mg Niacin, 0.0625 mg Niacinamide, 0.0625 mg p-Aminobenzoic acid, 0.125 mg Pyridoxal . HCl, 0.0625 mg Pyridoxine . HCl, 0.0625 mg Riboflavin, 0.025 mg Thiamine . HCl, 0.025 mg Vitamin A (crystallized alcohol), 0.250 mg Vitamin B12, 10.000 mg Distilled water, 1.0 L *Some lots of Casein Digest, Yeast Extract, or serum may inhibit growth.

Growth Conditions

Temperature: 25.0°C

Duration: axenic; anaerobic

Name of Depositor	LS Diamond
Year of Origin	1959
References	Diamond LS. A new Monocercomonas from the water snake, Natris sipedon. J. Protozool. 6: 8, 1959. Gunderson J, et al. Phylogeny of trichomonads inferred from small-subunit rRNA sequences. J. Eukaryot. Microbiol. 42: 411-415, 1995. PubMed: 7620466 Delgado-Viscogliosi P, et al. Electrophoretic mobility of tubulin subunits as a criterion for testing relationships between trichomonad taxa. Arch. Protistenkd. 146: 191-200, 1995. Viscogliosi E, et al. Phylogenetic implication of iron-containing superoxide dismutase genes from trichomonad species. Mol. Biochem. Parasitol. 80: 209-214, 1996. PubMed: 8892298 Keeling PJ, et al. Evolutionary relationship between translation initiation factor eIF-2gamma and selenocysteine-specific elongation factor SELB: change of function in translation factors. J. Mol. Evol. 47: 649-655, 1998. PubMed: 9847405 Viscogliosi E, Mueller M. Phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates. J. Mol. Evol. 47: 190-199, 1998. PubMed: 9694668 Gerbod D, et al. Phylogenetic relationships of class II fumarase genes from trichomonad species. Mol. Biol. Evol. 18: 1574-1584, 2001. PubMed: 11470849 Brugerolle G, et al. Immunolocalization of two hydrogenosomal enzymes of Trichomonas vaginalis. Parasitol. Res. 86: 30-35, 2000. PubMed: 10669133
Cross References	Nucleotide (GenBank) : U17511 SSU rRNA Nucleotide (GenBank) : Z70667 Monocercomonas sp. sod gene. Nucleotide (GenBank) : U17507 Monocercomonas sp. ATCC 50210 16S-like rRNA gene. Nucleotide (GenBank) : U66902 Monocercomonas ATCC50210 alpha-tubulin (atub1) gene, partial cds. Nucleotide (GenBank) : U66903 Monocercomonas ATCC50210 alpha-tubulin (atub2) gene, partial cds. Nucleotide (GenBank) : AF022420 Monocercomonas sp. glyceraldehyde-3-phosphate dehydrogenase (gap) gene, partial cds. Nucleotide (GenBank) : AF005077 Monocercomonas ATCC50210 translation initiation factor 2 gamma subunit (eIF-2 gamma) gene, partial cds.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Monocercomonas sp. (ATCC® 50210™)

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Monocercomonas sp. (ATCC^® 50210^™)