Phytomonas davidi Lafont (ATCC® 30287)

Depositor: RB McGhee  /  Biosafety Level: 1

Permits and Restrictions

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Biosafety Level 1
Isolation
Euphorbia heterophylla, Ocochobee City, FL, 1975
Product Format frozen
Type Strain no
Comments
Transmission and ultrastructure
conservation of a 29-base-pair sequence within maxicircle ARS fragments
Unique no-branched polyunsaturated fatty acids
Cyclopropane fatty acid
isoleucine requirement and threonine deaminase
Medium Medium 355: Crithidia medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor RB McGhee
Year of Origin 1975
References

. . J. Protozool. 22: 10A, 1975.

Postell FJ, McGhee RB. Phytomonas davidi (Trypanosomatidae): transmission and ultrastructure. J. Protozool. 22: 10A, abstract 9, 1975.

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Kim R, Ray DS. Conservation of a 29-base-pair sequence within maxicircle ARS fragments from six species of trypanosomes. Gene 40: 291-299, 1985. PubMed: 3938423

Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116

Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation