Blastocrithidia leptocoridis (McCulloch) Wallace and Dyer (ATCC® 30265)

Organism: Blastocrithidia leptocoridis (McCulloch) Wallace and Dyer  /  Depositor: FG Wallace

Biosafety Level 1
Isolation
water strider, Leptocoris trivittatus, Minneapolis, MN, 1960
Product Format frozen
Type Strain yes
Medium Medium 807: Brain heart infusion blood agar
Growth Conditions
Temperature: 30.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace
Year of Origin 1960
References

. . J. Parasitol. 48: 32, 1962.

type strain