Herpetomonas muscarum ingenoplastis Rogers and Wallace (ATCC® 30259)

Organism: Herpetomonas muscarum ingenoplastis Rogers and Wallace  /  Depositor: FG Wallace, WE Rogers

Biosafety Level 1
Isolation
Phormia regina, Minneapolis, MN, 1968
Product Format frozen
Type Strain yes
Comments
species description
Unique no-branched polyunsaturated fatty acids
Cyclopropane fatty acid
Medium ATCC® Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 20.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace, WE Rogers
Year of Origin 1968
References

Rogers WE, Wallace FG. Two new subspecies of Herpetomonas muscarum (Leidy, 1856) Kent, 1880. J. Protozool. 18: 645-654, 1971. PubMed: 4109037

Yoshida N. Herpetomonas mariadeanei sp. n. (Protozoa, Trypanosomatidae) from Muscina stabulans (Fallen, 1816) (Diptera, Muscidae). J. Protozool. 25: 421-425, 1978.

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937

type strain