Leptomonas seymouri Wallace (ATCC® 30220)

Depositor: FG Wallace  /  Biosafety Level: 1

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Biosafety Level 1
Isolation
Dysdercus suturellus, Stuart, FL, 1959
Product Format frozen
Type Strain no
Comments
species description
Trypanosomatids from fruit
Distribution of carbohydrate epitopes
Proteolytic activities
Acetylornithinase and ornithine acetyltransferase
isoenzyme electrophoresis for species identification
Cyclopropane fatty acid
enzymes of ornithine-arginine metabolism
Inhibition by antiprotozoal drugs
Cysteine and metalloproteinase activities
Monoclonal antibodies for identification
isoleucine requirement and threonine deaminase
conservation of 29-base-pair sequence within maxicircle ARS fragments
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace
Year of Origin 1959
References

Wallace FG. Leptomonas seymouri sp. n. from the cotton stainer Dysdercus suturellus. J. Protozool. 24: 483-484, 1977. PubMed: 599500

Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989.

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989.

Teixeira MM, et al. Characterization of the target antigens of Phytomonas-specific monoclonal antibodies. J. Eukaryot. Microbiol. 42: 232-237, 1995.

Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943

Bacchi J, et al. Susceptibility of an insect Leptomonas and Crithidia fasciculata to several established antitrypanosomatid agents. Antimicrob. Agents Chemother. 6: 785-790, 1974. PubMed: 4451351

Bacchi CJ, et al. Polyamines in trypanosomatids. J. Bacteriol. 131: 657-661, 1977. PubMed: 885842

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594

Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes. J. Protozool. 26: 648-652, 1979. PubMed: 161788

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Kim R, Ray DS. Conservation of a 29-base-pair sequence within maxicircle ARS fragments from six species of trypanosomes. Gene 40: 291-299, 1985. PubMed: 3938423

Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism. Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116

Goldberg B, et al. Inhibition by several standard antiprotozoal drugs of growth and O2 uptake of cells and particulate preparations of a Leptomonas. J. Protozool. 21: 322-326, 1974. PubMed: 4365631

Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198

Cross References

Nucleotide (GenBank) : X07487 Leptomonas seymouri mini-exon donor RNA (medRNA).

Nucleotide (GenBank) : X78552 L.seymouri (ATCC 30220) U6 snRNA, tRNA-Ile and tRNA-Gln genes.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation