Naegleria italica De Jonckheere (ATCC® PRA-152)

Organism: Naegleria italica De Jonckheere  /  Depositor: JF De Jonckheere

Permits and Restrictions

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Strain Designations N 243
Biosafety Level 2
Isolation
freshwater
Pantano Villa Peru
Isolation date: February, 2004
Product Format frozen
Type Strain no
Growth Conditions
Temperature: 37.0°C
Duration: monoxenic; non-nutrient agar with E. coli
Cryopreservation

1.   Allow the cells to encyst.  To detach cysts from the plate flush the surface with 5 ml fresh ATCC® medium 1323 (Page's Balanced Salt Solution).  Rub the surface of the plate with a spread bar to detach adhering amoebae.

2.   Transfer the cyst suspension to a sterile centrifuge tube.

3.   If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration.  To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106.

4.   While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:  Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.  Allow the DMSO to solidify.  Add the required volume of refrigerated medium.  Dissolve the DMSO by inverting the tube several times. 

Name of Depositor JF De Jonckheere
Year of Origin February, 2004
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation