Herpetomonas sp. 2 (ATCC® 50392)

Organism: Herpetomonas sp. 2  /  Depositor: CP McHugh

Strain Designations McHugh1
Biosafety Level 1
Isolation
Culicoides sp. reared from cactus collected in San Antonio, Bexar County, TX, 1993.
Product Format frozen
Type Strain no
Comments
This species is unique since it does not have an emergent flagellum.
Medium Medium 807: Brain heart infusion blood agar
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor CP McHugh
Year of Origin 1993