Leishmania donovani (Laveran and Mesnil) Ross (ATCC® 30626)

Organism: Leishmania donovani (Laveran and Mesnil) Ross  /  Depositor: RG Yaeger

Strain Designations Malakal
Application
Vector borne research
Biosafety Level 2
Isolation
human male, Malakal, Sudan, 1964
Product Format frozen
Type Strain no
Comments
Amastigotes.
Growth Conditions
Duration: in vivo, hamster
Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCC medium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum.
Subcultivation
Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCC medium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum.
Name of Depositor RG Yaeger
Chain of Custody
ATCC <<--RG Yaeger<<--P. Manson-Bahr <<--- J. Dempsey
Year of Origin 1964