Herpetomonas sp. (ATCC® 30598)

Organism: Herpetomonas sp.  /  Depositor: RB McGhee

Biosafety Level 2
Isolation
human, female liver biopsy, Corpus Christi, TX, 1976
Product Format frozen
Type Strain no
Comments
Unique no-branched polyunsaturated fatty acids
Cyclopropane fatty acid
Medium Medium 1011: Diphasic blood agar medium
Medium 1011: Diphasic blood agar medium
Medium 947: L diphasic blood agar (ATCC Medium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 ml
Growth Conditions
Max Temperature: 37.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor RB McGhee
Chain of Custody
ATCC <<--RB McGhee<<--CDC <<--- Spohn Hospital
Year of Origin 1976
References

McGhee RB, Cosgrove WB. Biology and physiology of the lower Trypanosomatidae. Microbiol. Rev. 44: 140-173, 1980. PubMed: 6997722

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937