Leptomonas collosoma Wallace et al. (ATCC® 30261)

Depositor: FG Wallace  /  Biosafety Level: 1

Permits and Restrictions

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Biosafety Level 1
Isolation
Gerris dissortis, Minneapolis, MN, 1960
Product Format frozen
Type Strain yes
Comments
Trypanosomatids from fruit
Distribution of carbohydrate epitopes
Two new species from genus Gerris
presences in feces of lizards fed culture forms of the flagellates
RNA editing
Proteolytic activities
enzymes of ornithine-arginine metabolism
Monoclonal antibodies for identification
Acetylornithinase and ornithine acetyltransferase
Ultrastructural differences between species with and without endosymbionts
Cyclopropane fatty acid
Medium Medium 1011: Diphasic blood agar medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor FG Wallace
Year of Origin 1960
References

Wallace FG, et al. Two new species of flagellates cultivated from insects of the genus Gerris. J. Protozool. 7: 390-393, 1960.

Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989.

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989.

Dollahon NR, et al. Herpetomonas megaseliae, Crithidia fasciculata, and Leptomonas collosoma (Kinetoplastida, Trypanosomatidae) in feces of lizards fed culture forms of the flagellates. J. Protozool. 30: 58-62, 1983.

Maslov DA, et al. Evolution of RNA editing in kinetoplastid protozoa: pan-editing preceded 5'-editing. Nature 368: 345-348, 1994. PubMed: 8127370

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533

Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism. Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593

Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925

type strain

Cross References

Nucleotide (GenBank) : U05812 Herpetomonas muscarum ATCC 30261 kinetoplast (MURF1) and apocytochrome B (cytB) genes, partial cds, and ATPase subunit 6 (MURF4) gene, complete pre-RNA-edited DNA sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation